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A useful tip if you're going to be doing this: Before pc'ing the LC jar, align the hole with any distinguishable feature in the jar, be it the front of the jar where is says "Ball" or whatever.

These days I'm also back into LCs, thanks to grainwater.

The broth will be darker but that's not a problem for me.

2020-01-10 13:08:46 Region: The Ad Standard region to which this site has been assigned.

The most common LC teks tell you to build a special lid for the vessel.

Remember, we sterilized the LC jar placing the lid with the rubber side up, so when you take off the ring do it slowly and carefully while holding down the SFD with one finger, otherwise the ring might catch the SFD.

This operation should be done in less than 2 seconds, the needle should not touch anything and the jet of water has to hit the liquid, never the glass.

Also, the biopsy takes its sweet time to show growth on agar, whereas it grows rather quickly in a nutrient liquid medium, so why not shoot the biopsy straight into the broth to shave off some time and steps? As you can see, growth looks disorganized, like a streaked plate from spores would.

Boilling the water is not necessary.

Skip it at your own risk.

i spawned a few shoeboxes and 2 20qt minis.

I was recently informed that blunt needles also work for this but the sample taken with it appears to be bigger, which in my opinion is a disadvantage.